Experimental Pathological Study of Acute African Swine Fever

To study the clinicopathology and histopathology of African swine fever (ASF), and to explore the internal relationship between pathological changes and disease occurrence and development and its pathological mechanism, 13 Landrace pigs with bodyweight about 20 kg were intramuscular injected with African swine fever virus (ASFV), strain Pig/HLJ/18 at a dose of 10²HAD₅₀·mL^-1. During the experiment, all the dead pigs were systematically dissected and sampled, paraffin sections were produced, and haematoxylin-eosin staining was performed. Clinicopathological evaluation standards for acute ASF were established, then pathological lesions (classification variables) were expressed by counting frequency and percentage, and the lesion degree (continuous variables) was graded and scored according to different pathological changes of various tissues and organs. The results showed that all infected pigs were in line with the clinical characteristics of ASF, including acute, febrile and highly infectious, with a 100% incidence rate and 100% mortality. The dead pigs showed typical characteristics of septicemia, cadavers prone to corruption, blood clotting adverse or hemolysis, rigor mortis incomplete. The main pathological lesions were hemorrhagic necrotizing lymphadenitis, acute inflammatory splenomegaly (septic spleen), cerebral edema, pulmonary edema and lung consolidation et al. The spleen and lymphonodus are the target organs attacked by ASFV, with the most significant lesions, the earliest occurrence time, the longest duration and the highest frequency. The most prominent pathological changes are blood circulation disorders, including multiple pathological manifestations such as edema, hyperemia, congestion, hemorrhage, infarction, disseminated intravascular coagulation (DIC), and the most important characteristics are hemorrhagic lesions. The inflammatory reaction of lymphocytic exudation caused by ASFV runs through the whole process, especially in the middle and later stages of the course. The results suggest that the main pathological process of acute African swine fever is a typical immune/inflammatory cascade reaction and severe systemic blood circulation disorder, which resulted in the high incidence rate and high mortality rate of acute ASF.     

茄子新品种驻茄15号的选育

驻茄15 号是以自交系P04-11 为母本，以高代自交系驻Q-25 为父本配制而成的中早熟绿茄一代杂种。植株生长势强，果实卵圆形，纵径15.0 cm 左右，横径10.6 cm 左右，平均单果质量0.52 kg，果皮绿色、光亮，肉质硬度中等，商品性好，一般每667 m² 前期产量1 100 kg 左右，总产量5 300 kg 左右，田间对青枯病、绵疫病、黄萎病的抗性强于对照郑研早青茄，适宜河南省及周边省份春、秋设施及露地栽培。     

贵州喀斯特地区牧草引种试验评价

对国内外引进的6个禾本科牧草品种,9个豆科牧草品种以及喀斯特地区的4个对照品种,在贵州独山地区进行引种试验。结果表明：各参试品种生长良好,能安全越冬、越夏,一年可刈割5～6次,鲜草产量达36558．2～66450．0kg／hm2,干草产量10771．0～19869．0kg／hm2。综合性状表现较好的依次为鸭茅Totter,Wana,Tuoa;多年生黑麦草Nui,Bronsys;白三叶Alice,C6151,Prop,Pitua,Sustain;百脉根Mareb,Lel。     

鸡骨型白血病免疫琼扩与放射摄片诊断的比较

为了探索特异性免疫学诊断方法对鸡骨型白血病(OP)的检测效果。用双盲法分批采样同时进行羽髓琼脂扩散试验和胫骨X线摄片检查对比,共检834只鸡(433羽属天然自发OP鸡群,401羽属人工诱发实验鸡群)。结果羽髓琼扩试验的OP阳性检出率为3．48％(29／834：)。阳性病理符合率为75．86％(22／29)。阳性误诊(假阳性)率24．13％(7／29),阴性误诊(假阴性)率36．15％(291／805)。而X线摄片检查的OP阳性检出率为38．37％(320／834)。X线阳性完全可获病理组织学证实,不存在假阳性或假阴性。把禽白血病羽髓琼扩试验试用于检测鸡骨型白血病尚属初次报道,其阳性检出率低而误诊率高,尚不宜作OP检测手段。放射摄片诊断的阳性检出率高而误诊率低,可快速诊断,故适用于OP检疫。     

加系双肌臀大白猪不同杂交组合性能比较试验

本试验选择加系双肌臀大白猪、美系杜洛克、丹麦长白三个品种的公母猪,组成5个杂交组合(双公×双母,双公×长母,双公×杜母,杜公×双母),测定了双×双、长×双、双×长、双×杜、杜×双五个组合猪的繁殖性能与肥育性能。结果表明:杜×双组合在日增重(896.05g)、饲料报酬(2.80∶1)、瘦肉率(66.01%)等主要指标上优于其它杂交组合,且繁殖性能居中、胴体品质优良,是加系双肌臀大白猪二元杂交瘦肉猪生产中的最佳杂交父本品种。试验还表明,双肌臀大白猪产仔多(12.08头)、增重快(851.97g)、瘦肉率高(65.24%)、适应性强,值得大力繁殖推广。     

Non-steroidal anti-inflammatory drugs induce apoptosis in implanted hepatoma of mice

AIM: To study the mechanism of the effect of NSAIDs on apoptosis in mice hepatoma at anti-inflammatory doses. METHODS: Kunming breed mice were inoculated subcutaneously in the flank with mice hepatoma H22 cell line. The effects of ibuprofen, indomethacin, and nimesulide on apoptosis were determined by using electron microscopy, agarose gel electrophoresis, flow cytometry, and Western blot analysis of the expression of c-myc, bcl-x_L and bcl-2 proteins. RESULTS: NSAIDs induced apoptosis of mice implanted hepatoma, which includes the morphological changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the "ladder pattern" revealed by agarose gel electrophoresis of DNA. The apoptotic index was increased to 15%±1.0%, 29.7%±1.5%, 46.3%±3.5% from 3.3%±0.6% by detecting Sub-G₁ peaks on flow cytometry. Western blot analysis showed that levels of bcl-2 and bcl-x_L were significantly reduced by treatment with nimesulide. Ibuprofen and indomethacin decreased bcl-2 expression but increased bcl-x_L expression. C-myc wasn't changed in these groups. CONCLUSION: These results suggest that NSAIDs induces apoptosis of mice hepatoma, which may be due to their regulation on the expressions of bcl-2 family genes.     

2002年我国小麦条锈病发生回顾

2002年小麦条锈病是继 1950年、1964年和 1990年后在全国范围内又一次大流行 ,其发生面积近 670万hm²,损失小麦约 10亿kg。发生区域涉及甘、陕、川、渝、云、贵、宁、鄂、豫、鲁、冀等 11个省 (市 )区。 2001年冬季和2002年春季气候适宜 ,以条中 32号小种为代表的毒性菌系的发展 ,使我国绝大部分主栽小麦品种不抗病 ,出现了条锈病发生早、发展快和发生重等特点 ,促成了 2002年我国小麦条锈病的流行。尽管 2002年条锈病发生范围广 ,但损失少于 1990年。提出了以品种抗病性利用及小麦抗病性变异和病菌群体动态监测、药剂和农业防治相结合 ,病害发生流行预测预报和条锈菌菌源基地治理为主的持续治理策略。     

《离骚》求“女”喻“求君“说证微

求女”之喻旨历来是《离骚》研究的关键所在,”求贤”、“求君”之争至今尚无定论。治骚大家金开诚先生就曾明文指证“求女”乃喻求贤”。但本文作者立足于《离骚》本体,从创作旨意、主要内容、文章结构以及逻辑学等角度,多方求证了”求女”喻“求君”一说。     

被毛突变小鼠解剖及繁殖学特性观察

对一种新的具有个毛、无毛、稀毛３种表型的被毛突变小鼠的大体解剖学特性研究结果表明,其心肝、脾、肺、肾、肾上腺等脏器的位置、形态及组织学结构无表型间差异,但无毛小鼠被毛和皮肤结构异常,大多数脏器的重量和脏器系数表现出表型差异和性别差异（Ｐ〈０．０５或Ｐ〈０．０１）。繁殖学特性研究结果表明,虽然无毛小鼠雌雄生殖系统的器官组成、发情周期与其他２种表型之间无明显差异,但其雄性的初情期明显滞后（约２周左右）     

猪繁殖和呼吸综合症山东分离株ORF7基因的克隆、测序及鉴定

山东某些养殖场发生以母猪流产或产死胎、木乃伊等为特症的流行性繁殖障碍病,怀疑为猪繁殖与呼吸综合症病毒(PRRSV)所致.自行设计了一对针对PRRSV的N基因的特异性引物P1和P2,通过RT-PCR技术对分别从潍坊和济南收集到的可疑病料进行检测,结果为阳性,并得到1条366bp的DNA片段.纯化此扩增产物,然后与PMD-18T载体连接,转化大肠杆菌DH5α.结果得到了1个ORF7基因与PMD-18T载体的重组质粒PMD-18T-ORF7.通过EcoRⅠ/BamHⅠ双酶切及PCR证明此重组质粒即为ORF7基因与PMD-18T载体的重组质粒,从而为ORF7基因及其表达的核衣壳蛋白进一步研究奠定了基础.